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KMID : 0613820100200030403
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Journal of Life Science
2010 Volume.20 No. 3 p.403 ~ p.410
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Characterization of Bacillus licheniformis KJ-9 Isolated from Soil
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Seo Dong-Cheol
Ko Jeong-Ae
Gal Sang-Wan
Lee Sang-Won
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Abstract
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In order to produce high-quality fermenting composts, a microorganism was isolated from the natural world. The bacterium has not only in high enzyme activities but also had good antimicrobial activities against phytopathogenic microorganisms. Its cultivating characteristics were then investigated. Bacterium KJ-9, which contains high CMCase, protease and chitinase activities and excellent antimicrobial activities against phytopathogenic microorganisms, was separated from leaf mold and identified as Bacillus licheniformis by two methods: Bergey’s Manual of Systematic Bacteriology and API 50 CHL Carbohydrate Test Kit (Bio Merieux, France) using an ATB (Automated Identification) computer system (Bio Merieux, France). Optimal medium for cultivation of B. licheniformis was 2% soluble starch as a carbon source, 0.5% yeast extract as a nitrogen source and 0.05% MgSO₄ㆍ7H₂O. Optimal growth conditions of pH, temperature and shake speed were pH 7.0, 50℃and 180 rpm, respectively. Culture broth of B. licheniformis KJ-9 cultured for 36~60 hr was effective in fungicidal activities against plant pathogens including Botrytis cinerea, Corynespora cassicola, Fusarium oxysporum, and Rhizoctonia solani.
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KEYWORD
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Antimicrobial activity, Bacillus licheniformis, phytopathogenic microorganisms, optimalmedium
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